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It pro- duces new proteins or variations of proteins that may increase the survival rate of the species buy zenegra 100 mg on-line erectile dysfunction 17. Genetic change is produced by unrepaired mutations and by a mechanism known as recombination in which portions of chromosomes are exchanged purchase zenegra 100mg with mastercard impotence def. When she returns for a checkup, she asks whether such a large number of pills are really necessary for treatment of acquired immunodeficiency syndrome (AIDS). Di Abietes responded to treatment for her diabetes mellitus but subse- quently developed a low-grade fever, an increase in urinary urgency and frequency, and burning at the urethral opening with urination (dysuria). A urinalysis showed a large number of white blood cells and many Gram-negative bacilli. A urine culture indicated many colonies of Escherichia coli, which is sensi- tive to several antibiotics, including the quinolone norfloxacin. Melvin (Mel) Anoma is a 46-year-old man who noted a superficial, brown- ish-black, 5-mm nodule with irregular borders in the skin on his chest. He was scheduled for outpatient surgery, at which time a wide excision biopsy was performed. Examination of the nodule indicated histologic changes characteristic of a malignant melanoma reaching a thickness of only 0. Nick O’Tyne is a 62-year-old electrician who has smoked two packs of Replication forks cigarettes a day for 40 years. He recently noted that his chronic cough had gotten worse. His physician ordered a chest radiograph, which showed a 2-cm nodule in the upper lobe of the right lung. Cytologic study of the sputum by Papanicolaou technique showed cells consistent with the presence of a well- differentiated adenocarcinoma of the lung. DNA SYNTHESIS IN PROKARYOTES Parental strands The basic features of the mechanism of DNA replication are illustrated by the processes occurring in the bacterium E. This bacillus grows symbiotically in the human colon. It has been extensively studied and serves as a model for the more com- plex and, consequently, less well-understood processes that occur in eukaryotic cells. Replication Is Bidirectional Replication of the circular, double-stranded DNA of the chromosome of E. Synthesis begins at the origin and occurs at two replication forks that move away from the origin bidirectionally (in both directions at the same time). Replication ends on the other side of the chro- mosome at a termination point. One round of synthesis, involving the incorporation of over 4 million nucleotides in each new strand of DNA, is completed in approxi- mately 40 minutes. However, a second round of synthesis can begin at the origin Fig. Bidirectional replication of a circu- before the first round is finished. These multiple initiations of replication allow bac- lar chromosome. Replication begins at the terial multiplication to occur much more quickly than the time it takes to complete point of origin (oriC) and proceeds in both a single round of replication. Replication Is Semiconservative topoisomerase Each daughter chromosome contains one of the parental DNA strands and one newly synthesized, complementary strand. Therefore, replication is said to be semi- Unpaired bases conservative; i. Each one is paired with a newly synthesized strand (see Figs. Unwinding of Parental Strands Single-strand binding proteins Replication requires separation of the parental DNA strands and unwinding of the Fig. Proteins involved in separating and helix ahead of the replication fork. Helicases (DnaB) separate the DNA strands and unwinding parental DNA strands at the repli- unwind the parental duplex. Single-strand binding proteins prevent the strands from cation fork in prokaryotes. Topoisomerases, enzymes that can break phosphodiester bonds and rejoin them, relieve the supercoiling of the parental duplex caused by unwinding.

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When a container of blood is spun rapidly purchase zenegra 100 mg with visa erectile dysfunction age young, all the formed Checkpoint 13-13 What are the blood antigens most often in- elements of the blood are pulled into a clump at the bot- volved in incompatibility reactions? They are thus separated from the plasma buy cheap zenegra 100 mg impotence caused by medications, which is less dense. The formed elements may be further separated and used for specific purposes, for ex- ample, packed red cells alone or platelets alone. The gen- Blood can be packaged and kept in blood banks for emer- eral term for this procedure is hemapheresis gencies. To keep the blood from clotting, a solution such (hem-ah-fer-E-sis) (from the Greek word apheresis mean- as citrate-phosphate-dextrose-adenine (CPDA-1) is ing “removal”) If the plasma is removed and the formed added. Blood banks usually have all Use of Plasma Blood plasma alone may be given in an types of blood and blood products available. It is impor- emergency to replace blood volume and prevent circula- tant that there be an extra supply of type O, Rh-negative tory failure (shock). Plasma is especially useful when blood because in an emergency this type can be used for blood typing and the use of whole blood are not possible, any patient. It is normal procedure to test the recipient such as in natural disasters or in emergency rescues. Plasma separated from the cellular ele- gery if needed. This practice eliminates the possibility of ments is usually further separated by chemical means into incompatibility and of disease transfer as well. Such autol- various components, such as plasma protein fraction, ogous (aw-TOL-o-gus) (self-originating) blood is stored serum albumin, immune serum, and clotting factors. The packaged plasma that is currently available is ac- tually plasma protein fraction. Further separation yields Whole Blood Transfusions serum albumin that is available in solutions of 5% or 25% The transfer of whole human blood from a healthy person concentration. In addition to its use in treatment of cir- to a patient is often a life-saving process. Whole blood culatory shock, these solutions are given when plasma THE BLOOD 273 proteins are deficient. They increase the osmotic pressure death usually results. If the loss is gradual, however, over of the blood and thus draw fluids back into circulation. Possi- creased because these blood components can be treated ble causes of chronic blood loss include bleeding ulcers, with heat to prevent transmission of viral diseases. If the cause of the blood loss can be corrected, the minister fluids known as plasma expanders. These are body is usually able to restore the blood to normal. This cell-free isotonic solutions used to maintain blood fluid process can take as long as 6 months, and until the blood volume to prevent circulatory shock. When frozen plasma is thawed, a white precipitate called cryoprecipi- Hemolytic anemia Anemia caused by the excessive de- tate (kri-o-pre-SIP-ih-tate) forms in the bottom of the struction of red cells is called hemolytic (he-mo-LIH-tik) container. Plasma frozen when it is less than 6 hours old anemia. The spleen, along with the liver, normally de- contains all the factors needed for clotting. Occasionally, an overactive spleen de- tate is especially rich in clotting factor VIII and fibrino- stroys the cells too rapidly, causing anemia. These components may be given when there is a spe- may also cause red cell loss. For example, the malarial cial need for these factors. Certain inherited diseases that cause the production Antibodies play an important role in the immune system of abnormal hemoglobin may also result in hemolytic (see Chap.

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Fight-or-flight response mediated by brain: hypothalamus purchase zenegra 100mg erectile dysfunction treatment jaipur, trients in blood order zenegra 100mg overnight delivery impotence specialists; e. Hormones help body, meet stressful situations; can be balance; e. Unmanaged stress can be harmful; stress management E. Pancreas—islet cells of pancreas secrete hormones techniques help maintain overall health 1. Lack or poor cellular response causes diabetes melli- A. Aging-associated changes linked with endocrine system tus changes—loss of muscle and bone tissue (1) Type I—requires insulin B. Main clinical conditions associated with endocrine system (2) Type II—may be controlled with diet and exer- involve pancreas, thyroid cise 1. Adult-onset diabetics mellitus (3) Gestational—occurs curing pregnancy 2. Sex glands—needed for reproduction and development of menopause—only common treatment for age-associated secondary sex characteristics endocrine failure 260 CHAPTER TWELVE Questions for Study and Review Building Understanding Fill in the blanks 1. Chemical messengers carried by the blood are called 4. Local hormones active in promoting inflammation are 2. The part of the brain that regulates endocrine activity . Red blood cell production in the bone marrow is stim- ulated by . Matching Match each numbered item with the most closely related lettered item. A disorder caused by overproduction of growth hormone in the adult. A disorder caused by underproduction of parathyroid hormone. A disorder caused by underproduction of thyroid hormone. A disorder caused by overproduction of growth hormone in the child. A disorder caused by underproduction of antidiuretic hormone. A target tissue responds to a hormone only if it the hormones released from each division and describe has the appropriate the effects of each. Uterine contractions and milk ejection are promoted by d. Name the hormone released by the thymus gland; by 14. Epinephrine and norepinephrine are released by the pineal body. Sleep-wake cycles are regulated by the to the hospital after collapsing at school. Jefferson has rheumatoid arthritis, which is being Understanding Concepts treated with glucocorticoids. With regard to regulation, what are the main differ- his doctor notices that Mr. Jefferson’s face is “puffy” and ences between the nervous system and the endocrine sys- his arms are bruised. Explain how the hypothalamus and pituitary gland regulate certain endocrine glands. This life-giving fluid brings by means of substances (mainly proteins) that maintain nutrients and oxygen to the cells and carries away waste. Recall that osmotic pres- The heart pumps blood continuously through a closed sure is related to the concentration of dissolved and system of vessels. The heart and blood vessels are de- suspended materials in a solution.

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